Correlation between Hypo-osmotic Swelling Test and Various Conventional Semen Evaluation Parameters in Fresh Nili-ravi Buffalo and Sahiwal Cow Bull Semen

The present study was conducted to determine the correlation of hypo-osmotic swelling test with conventional semen evaluation parameters of fresh semen collected from two Nili-Ravi buffalo and two Sahiwal cow bulls. A total of 10 pooled samples (each comprising two consecutive ejaculates) from each bull were collected. Each semen sample was divided into two parts. One part was used for the evaluation of semen by conventional method, while the other part was subjected to hypo-osmotic swelling test by using 150 mOsm/L sodium citrate fructose solution. The mean sperm positive to HOS test was 85.25% both in Nili-Ravi buffalo and Sahiwal cow bull semen. Statistical analysis of the data revealed a significant (P<0.05) positive correlation between progressive motility, morphologically normal spermatozoa, sperm viability and percentage of HOS test positive spermatozoa for both species. It was inferred that HOS test could be a valuable method for routine evaluation of semen for artificial insemination.